Lipoeasy® is Harmony Tao's proprietary lipid-based transfection reagent. It adapts to different LNP formulations and efficiently transfects nucleic acid cargo — mRNA, siRNA, plasmid DNA — with high encapsulation efficiency, positioning it as a highly customisable drug delivery solution.
对敏感细胞与动物模型友好，HEK293 / DC2.4 在 0.1–5 μg/mL pDNA 区间细胞活率接近 100%。
Friendly to sensitive cells and animal models: HEK293 and DC2.4 maintain near-100% viability across 0.1–5 μg/mL pDNA.
无需配制 LNP、无需微流控设备，一步完成核酸包封。
No LNP formulation set-up, no microfluidics — nucleic acid encapsulation in one step.
同一试剂覆盖细胞转染与动物给药（IV、IM 等）两条验证路径。
One reagent covering cell transfection and animal administration (IV, IM and more).
以更低的试剂与设备门槛完成临床前递送验证。
Preclinical delivery validation with a lower reagent and equipment threshold.
以下图表均直接来自产品说明材料，未做数值清洗或推演。
All charts below are taken directly from the product documentation, without numerical cleaning or extrapolation.
在 HeLa、HEK293、Hep2、NCI-H460、COLO 细胞上，Lipoeasy® 与 Lipoeasy®-Pro 的转染效率持续优于 Lipo-fectamine 3000，显示跨细胞类型的广泛适用性（红色荧光为表达信号）。
Across HeLa, HEK293, Hep2, NCI-H460 and COLO cells, Lipoeasy® and Lipoeasy®-Pro consistently outperformed Lipo-fectamine 3000 — broad applicability across cell types (red fluorescence indicates expression).
肌肉注射给药后，Lipoeasy® 递送的 Luc-mRNA 与 LNP 递送呈现相当的报告基因表达谱，注射部位荧光素酶表达无统计学显著差异。
After intramuscular administration, Luc-mRNA delivered by Lipoeasy® shows a protein expression profile comparable to LNP delivery, with no statistically significant difference in luciferase expression at the injection site.
在 HEK293 与 DC2.4 细胞上，pDNA 浓度 0.1–5 μg/mL 全区间内 Lipoeasy® 细胞活率维持在接近 100%，提示极低的细胞毒性反应。
On HEK293 and DC2.4 cells, across the full 0.1–5 μg/mL pDNA range, Lipoeasy® maintained cell viability at nearly 100% — indicating a very low cytotoxic response.
红色荧光为报告基因表达信号，逐细胞系对比 Lipoeasy / Lipoeasy-Pro 与 Lipo-fectamine 3000。
Red fluorescence shows reporter expression, comparing Lipoeasy / Lipoeasy-Pro against Lipo-fectamine 3000 cell by cell.
| Lipofectamine 3000 | in vivo fectamine™ 3.0 | in vivo-jetPEI® | in vivo-jetRNA®+ | LipidBrick® IM21.7c | Entranster™-in vivo | Lipoeasy® | |
|---|---|---|---|---|---|---|---|
| 体外转染 / In vitro transfection | Y | N | N | N | N | N | Y |
| 体内转染 / In vivo transfection | N | Y | Y | Y | N | N | Y |
| 上样方式 / Loading method | Lipid coat | — | — | Lipid coat | — | — | self-assembly LNP |
| 载体类型 / Carrier type | LNP | LNP (non-animal source) | Non-LNP | Lipid nanopolymers | Lipid brick | Non-lipid nanopolymers | LNP |
| 支持处方优化 / Formulation optimisation | N | N | N | Y | Y | N | Y |
| 对接 LNP 商业化 / Link to LNP commercialisation | N | Y | N | N | N | N | Y |
| 供应商 / Supplier | ThermoFisher | ThermoFisher | Sartorius | Sartorius | Sartorius | Engreen | Harmony Tao |
Reproduced from the product documentation (Y = supported, N = not supported). Lipo-fectamine 3000 supports in vitro only, with no nucleic acid encapsulation; Lipoeasy® is the only product combining in vitro and in vivo transfection, encapsulated loading, formulation optimisation and a link to LNP commercialisation.
| Model | Pack size | Cargo | Cell types |
|---|---|---|---|
| Lipoeasy-Pro-01 | 32 mg × 1 / 32 mg × 3 | pDNA, siRNA, mRNA, circRNA, ASO, miRNA | 贴壁细胞 / 悬浮细胞 · Adherent / suspension |
| Lipoeasy-Pro-02 | 32 mg × 1 / 32 mg × 2 | pDNA, siRNA, mRNA, circRNA, ASO, miRNA | 贴壁细胞 / 悬浮细胞 · Adherent / suspension |
| Lipoeasy-01 | 32 mg × 1 / 32 mg × 3 | pDNA, siRNA, mRNA, circRNA, ASO, miRNA | 贴壁细胞 / 悬浮细胞 · Adherent / suspension |
| Lipoeasy-02 | 32 mg × 1 / 32 mg × 2 | pDNA, siRNA, mRNA, circRNA, ASO, miRNA | 贴壁细胞 / 悬浮细胞 · Adherent / suspension |
| Lipoeasy-target-01 | 32 mg × 1 | pDNA, siRNA, mRNA, circRNA, ASO, miRNA | 贴壁细胞 / 悬浮细胞 · Adherent / suspension |
如需技术资料、报价或试用样品，请通过合作洽谈页提交需求，或直接联系我们。
For technical documentation, quotations or trial samples, submit a request on the contact page or reach us directly.
